Lab Introduction & Major Research Interests
Prof. Tang-Long Shen received his BS and MS degrees from National Taiwan University in the Department of Plant Pathology in 1991 and 1994, respectively, and obtained his PhD from Cornell University in the Department of Molecular Medicine in 2002. After his postdoc training in American Heart Association, Prof. Shen joined the Department of Plant Pathology and Microbiology at the National Taiwan University since 2004. His research explores varied subjects, including microbes, plants to human, to understand the basic mechanisms of various pathologic complications as well as how to utilize the microbial metabolites or natural product for human health. By using molecular biology, biochemistry and cellular biology approaches as well as animal models, Prof. Shen deciphers the molecular mechanism of cancer progression, especially how cancer cells interact with tumor microenvironment in cell migration, the formation of pre-metastatic niches and metastatic organotropism. Several key targets, including integrins, receptor tyrosine kinases (e.g. EGFR), non-receptor tyrosine kinases (e.g. FAK, Src), adaptor proteins (e.g. Grb7, C35) and cytokines as well as extracellular vesicles (e.g. exosomes), are main focuses. Additionally, Shen lab is also seeking useful natural products and de novo protein components in human cells including critical secondary metabolites from traditional Chinese medicinal fungi, Cordyceps spp, Antrodia cinnamomea, and metallothioneins, to apply for anti-cancer, anti-aging or other therapeutics.
Recent Representative Publication ( * corresponding author)
1. Yu-Ling Tai, Pei-Yu Chu, I-Rui Lai, Hui-Yuan Tseng, Jun-Yang Liou, Jun-Lin Guan, Tang-Long Shen* (2015) An EGFR/Src-dependent b4 Integrin/FAK complex contributes to the malignancy of breast cancer. Scientific Reports 5, 16408; doi: 10.1038/srep16408
2. Pei-Yu Chu, Tsia-Kun Li, Shih-Torng Ding, I-Rue Lai, Tang-Long Shen* (2010) EGF-induced Grb7 activation promotes Ras activity essential for the tumorigenicity of Sk-Br3 breast cancer cells. Journal Biological Chemistry 285(38):29279-85
3. I-Rue Lai, Pei-Yu Chu, Hsiao-Sheng Lin, Jun-Yang Liou, Yee-Jee Jan, Jen-Chieh Lee, Tang-Long Shen* (2010) Autophosphorylation of Tyr-397 of focal adhesion kinase in gastric carcinoma and its clinical significance. American Journal of Pathology 177(4):1629-37 (This article is highlighted by Nature Reviews Gastroenterology and Hepatology 7, 592)
4. Bruno Costa-Silva, Nicole M. Aiello, Swarnima Singh, Haiying Zhang, Basant Kumar Thakur, Annette Becker, Ayuko Hoshino, Milica Tešić Mark, Henrik Molina, Jenny Xiang, Tuo Zhang, Till-Martin Theilen, Guillermo García-Santos, Caitlin Williams, Yonathan Ararso, Yujie Huang, Gonçalo Rodrigues, Tang-Long Shen, et. al. (2015) Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nature Cell Biol. 17(6):816-26.
5. Ayuko Hoshino*, Bruno Costa-Silva*, Tang-Long Shen*, et. al. (2015) Tumor exosome integrins determine organotropic metastasis. Nature 527(7578):329-35 (*co-first authors)
6. Yu-Ling Tai, I-Rue Lai, Yu-Ju Peng, Shih-Torng Ding, Tang-Long Shen* (2016) Activation of focal adhesion kinase through an interaction with β4 integrin contributes to colon cancer progression. FEBS letters 590(12):1826-37
7. Huang CW, Hong TW, Wang YJ, Chen KC, Pei JC, Chuang TY, Lai WS, Tsai
SH, Chu R, Chen WC, Sheen LY, Takahashi S, Ding ST, Shen TL* (2016) Ophiocordyceps formosana extract improves hyperglycemia and depression in an STZ-induced mouse model BMC Complementary and Alternative Medicine 16(1):310
8. Yu-Ling Tai, Li-Hsuan Tung, Yu-Chi Lin, Pei-Jung Lu, Pei-Yu Chu, Ming-Yang Wang, Wei-Pang Huang, Ko-Chien Lin, Hsinyu Lee, Tang-Long Shen＊ (2016) Grb7 Protein Stability Modulated by Pin1 in Association with Cell Cycle Progression. PLoS One 11(9):e0163617
9. Yu-Ling Tai, Ko-Chien Chen, Jer-Tsong Hsieh, Tang-Long Shen* (2018) Exosomes in cancer development and clinical applications. Cancer Science 109(8):2364-2374. doi: 10.1111/cas.13697.
10. Yueh-Chien Lin, Chien-Chin Chen, Wei-Min Chen, Kuan-Ying Lu, Tang-Long Shen, Yeong-Chin Jou, Cheng-Huang Shen, Norihiko Ohbayashi, Yasunori Kanaho, Yuan-Li Huang, Hsinyu Lee (2018) LPA1/3 signaling mediates tumor lymphangiogenesis through promoting CRT expression in prostate cancer. BBA – Molecular and Cell Biology of Lipids 1863(10):1305-1315.
11. Tai YL, Chu PY, Lee BH, Chen KC, Yang CY, Kuo WH, Shen TL＊. (2019) Basics and applications of tumor-derived extracellular vesicles. J Biomed Sci. 26(1):35.
12. Chu PY, Tai YL, Shen TL＊. (2019) Grb7, a Critical Mediator of EGFR/ErbB Signaling, in Cancer Development and as a Potential Therapeutic Target. Cells 8(5). doi: 10.3390/cells8050435.
13. Wu PY, Yu IS, Lin YC, Chang YT, Chen CC, Lin KH, Tseng TH, Kargren M, Tai YL, Shen TL, Liu YL, Wang BJ, Chang CH, Chen WM, Juan HF, Huang SF, Chan YY, Liao YF, Hsu WM, Lee H. (2019) Activation of Aryl Hydrocarbon Receptor by Kynurenine Impairs Progression and Metastasis of Neuroblastoma. Cancer Res. 79(21):5550-5562
14. Chun-Jung Lin, Eun-Jin Yun, U-Ging Lo, Yu-Ling Tai, Su Deng, Elizabeth Hernandez, Andrew Dang, Yu-An Chen, Debabrata Saha, Ping Mu, Ho Lin , Tsai-Kun Li, Tang-Long Shen, Chih-Ho Lai and Jer-Tsong Hsieh (2019) The Paracrine Induction of Prostate Cancer Progression by Caveolin-1. Cell Death & Disease 10(11):834. doi: 10.1038/s41419-019-2066-3.
15. Gao YL, Wang YJ, Chung HH, Chen KC, Shen TL, Hsu CC. Molecular networking as a dereplication strategy for monitoring metabolites of natural product treated cancer cells. (2019) Rapid Commun Mass Spectrom. doi: 10.1002/rcm.8549
16. Kuo TH, Chung HH, Chang HY, Lin CW, Wang MY, Shen TL, Hsu CC. (2019) Deep Lipidomics and Molecular Imaging of Unsaturated Lipid Isomers: A Universal Strategy Initiated by mCPBA Epoxidation. Anal Chem. 91(18):11905-11915. doi: 10.1021/acs.analchem.9b02667.
17. Chu PY, Tai YL, Shen TL. (2019) Grb7, a Critical Mediator of EGFR/ErbB Signaling, in Cancer Development and as a Potential Therapeutic Target. Cells. 8(5). pii: E435. doi: 10.3390/cells8050435.
18. Li-En Lin; Chih-Lin Chen; Ying-Chen Huang; Hsin-Hsiang Chung; Chiao-Wei Lin; Ko-Chien Chen; Yu-Ju Peng; Shih-Torng Ding; Ming-Yang Wang; Tang-Long Shen; Cheng-Chih Hsu (2019) Precision Biomarker Discovery Powered by High Spatial Resolution Ambient Ionization Mass Spectrometry Imaging Using Microscopy Image Fusion. Analytica Chimica Acta 1100:75-87
19. I-Chen Hung, Tsung-Ming Chen, Jing-Ping Lin, Yu-Ling Tai, Tang-Long Shen and Shyh-Jye Lee (2020) Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42. Open Biology 10(2) :190273
20. Tai YL, Lin CJ, Li TK, Shen TL, Hsieh JT, Chen BPC. (2020) The role of extracellular vesicles in prostate cancer with clinical applications. Endocr Relat Cancer. pii: ERC-20-0021.R1. doi: 10.1530/ERC-20-0021.